Unlocking the Potential for Petroleum Biodegradation with QuantArray®-Petro

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Unlocking the Potential for Petroleum Biodegradation with QuantArray®-Petro Comprehensive evaluation of biodegradation potential at petroleum impacted sites can by challenging due to two factors: (1) Petroleum products are complex mixtures of hundreds of aliphatic, aromatic, cyclic and heterocyclic compounds. (2) Even for common classes of contaminants like benzene, toluene, ethylbenzene, and xylenes (BTEX), biodegradation can…

Highlighting Min-Traps: Revolutionizing Monitoring to Document Abiotic Degradation

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Highlighting Min-Traps: Revolutionizing Monitoring to Document Abiotic Degradation The Min-Trap® sampler passively collects direct mineralogical data using existing monitoring wells. Many in-situ groundwater treatment technologies for chlorinated solvents, as well as many metals and other contaminants, rely upon the formation, dissolution, and/or transformation of target solid-phase minerals either to sequester or degrade groundwater contaminants. However, evaluation of…

Navigating Natural Source Zone Depletion with QuantArray®NSZD

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Navigating Natural Source Zone Depletion with QuantArray® NSZD   Natural Source Zone Depletion (NSZD) is a complex phenomenon that involves the natural reduction and removal of light non-aqueous phase liquid (LNAPL) petroleum hydrocarbons from the subsurface. Microbial Insights Inc. introduces QuantArray® NSZD, a revolutionary tool that uncovers the intricate microbial dynamics behind this process. Let’s…

Next generation sequencing (NGS) provides comprehensive identification of microorganisms present in a sample down to the genus and even species level.

Next Generation Sequencing (NGS): Characterization of Microbial Communities at a Pipeline Release

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• At a petroleum pipeline release in a remote area Bio-Traps® and CENSUS® qPCR for BTEX catabolic functional genes proved stimulation of BTEX biodegradation in impacted groundwater and provided an additional line of evidence to support acceptance of an MNA management strategy at the site.

• Contaminant trends, geochemistry, CENSUS® qPCR, and NGS provided multiple lines of evidence supporting an MNA management strategy and avoiding the greater costs of enhanced remediation.

SIP: Evaluating MNA at a Manufactured Gas Plant

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– Site stakeholders needed conclusive evidence that biodegradation of benzene and naphthalene was occurring under existing site conditions to accept MNA instead of a more aggressive strategy.

– Stable isotope probing (SIP) studies using Bio-Traps® amended with 13C benzene or 13C naphthalene conclusively demonstrated in situ biodegradation occurred under existing site conditions.

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QuantArray®-Petro: Effectiveness of Oxygen Addition at a Former Gas Station

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• Groundwater at a former gasoline service station was impacted by leaking underground storage tanks and associated piping. Although contaminant trends and geochemistry suggested that MNA could be an appropriate site management strategy, the need for more rapid site closure led to a decision to enhance biodegradation of BTEX and MTBE aerobically with injection of an oxygen releasing compound.

Featured Image for Census qPCR Actionable Data for Evaluating MNA Case Study

CENSUS® qPCR: Actionable Data for Evaluating MNA

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• A petroleum pipeline release in a remote area had gone undiscovered for a number of years and groundwater was impacted with BTEX. Groundwater monitoring results suggested that BTEX concentrations were decreasing although results were variable.

• Trends in decreasing BTEX concentrations were validated as biological in origin supporting an MNA management strategy and avoiding the greater costs of enhanced remediation.