Next Generation Sequencing (NGS) is a DNA based technique used to identify the microorganisms present in a sample, providing a profile of the microbial community. Comprehensive identification of the microorganisms present offers unprecedented insight into the potential for microbiologically influenced corrosion (MIC).
NEXT GENERATION SEQUENCING (NGS) MIC ADVANTAGES:
ACCURATE
Who is there? NGS provides comprehensive identification of the microorganisms present in a sample down to the genus and in some cases even the species level.
REVEALING
NGS reveals an overall profile of microbial community composition and the relative proportions of the classified microorganisms based on number of sequence reads.
DESCRIPTIVE
NGS reports include brief descriptions of the top genera identified in each sample.
COMPARATIVE
Reports include statistical analyses that visually compare NGS results between samples to reveal important differences or shifts in the microbial community by location, over time, or in response to treatment.
HOW TO USE NEXT GENERATION SEQUENCING (NGS) MIC:
NGS is most appropriate for identifying members of the microbial community present in a sample when little is known about the process in question. Results provide insight into the potential microbial activities and can be used to select CENSUS qPCR targets for routine MIC monitoring.
RESOURCES
CASE STUDIES
NGS: Characterizing Microbial Communities and Assessing MIC Threats
– This case study shows how NGS revealed diverse microbial communities and the presence of microbial groups commonly implicated in MIC, including SRB, methanogens and acetogens.