CENSUS qPCR (quantitative polymerase chain reaction) is a DNA based method to accurately quantify specific microorganisms (e.g. Dehalococcoides, Dehalobacter) and functional genes (e.g. vinyl chloride reductase, anaerobic benzene carboxylase) responsible for biodegradation of contaminants.

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CENSUS qPCR ER ADVANTAGES:

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ACCURATE

Direct analysis of sample DNA removes the need to grow the bacteria, thus eliminating biases associated with traditional approaches (e.g., plate counts and MPNs).

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QUANTITATIVE

Absolute quantification of the concentrations of specific microorganisms and functional genes encoding enzymes responsible for contaminant biodegradation gives site managers a direct line of evidence to evaluate remediation options and monitor remedy performance. Results reported as cells/mL, cells/g, etc.

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INFORMATIVE

Is that a low, medium, or high concentration of contaminant degraders? With the MI Database, clients can retrieve percentile rankings of their CENSUS®qPCR results to answer that question based on the tens of thousands of samples MI has received from sites around the world.

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SENSITIVE

Practical Detection Limits (PDL) are as low as 100 cells per sample with a dynamic range over seven orders of magnitude. Low detection limits are particularly important when evaluating whether bioaugmentation is needed or an unnecessary expense.

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SPECIFIC

Target specific bacterial groups (e.g., Dehalococcoides, Dehalobacter, Dehalogenimonas) and functional genes (e.g. vinyl chloride reductases, anaerobic benzene carboxylase) responsible for contaminant biodegradation.

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COST EFECTIVE

Fast turnaround time (7-10 days), with rush service available, so you can make decisions and take action quickly. CENSUS®qPCR is inexpensive and ultimately saves money by allowing site managers to make more informed decisions.

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FLEXIBLE

Analysis can be performed on almost any type of sample (water, soil, sediments, Bio-Traps®, and others).

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CUSTOMIZEABLE

Assays are available for quantification of many microorganisms and functional genes responsible for biodegradation of a broad spectrum of contaminants. Custom assays can also be developed to fit your needs.

HOW CENSUS qPCR ER WORKS:

Along with contaminant concentrations and geochemical parameters, the concentrations of specific microorganisms and functional genes responsible for contaminant biodegradation is a key component of remedy selection and performance monitoring at sites impacted by chlorinated solvents, petroleum hydrocarbons, and emerging contaminants.

Use CENSUS qPCR to help answer…

  • How feasible is MNA? Is enhanced bioremediation needed? Is bioaugmentation needed?
    • What are the concentrations of contaminant degraders under existing conditions?
    • Are degrader concentrations greater in impacted wells than background wells?
    • Based on the MI Database, are concentrations of contaminant degraders low, medium, or high in the plume?

  • Is enhanced bioremediation effective?
    • Did concentrations of contaminant degraders and functional genes increase in response to treatment?
    • Are additional types of degraders and functional genes now detected that were previously below detection limits?

RESOURCES

CASE STUDIES

CENSUS qPCR: Evaluating Biostimulation to Overcome DCE Stall

– Site managers were concerned that cis-DCE was accumulating at a chlorinated solvent site.
– CENSUS qPCR quantification of Dehalococcoides and functional genes was performed to evaluate the potential for complete reductive dechlorination to ethene and to assess enhanced bioremediation strategies.

FIND OUT MORE:
How CENSUS® qPCR Can Help With These Contaminants

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