CENSUS qPCR (quantitative polymerase chain reaction) is a DNA based method to accurately quantify specific microorganisms (e.g. Dehalococcoides, Dehalobacter) and functional genes (e.g. vinyl chloride reductase, anaerobic benzene carboxylase) responsible for biodegradation of contaminants.

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CENSUS qPCR ER ADVANTAGES:

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ACCURATE

Direct analysis of sample DNA removes the need to grow the bacteria, thus eliminating biases associated with traditional approaches (e.g., plate counts and MPNs).

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QUANTITATIVE

Absolute quantification of the concentrations of specific microorganisms and functional genes encoding enzymes responsible for contaminant biodegradation gives site managers a direct line of evidence to evaluate remediation options and monitor remedy performance. Results reported as cells/mL, cells/g, etc.

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INFORMATIVE

Is that a low, medium, or high concentration of contaminant degraders? With the MI Database, clients can retrieve percentile rankings of their CENSUS®qPCR results to answer that question based on the tens of thousands of samples MI has received from sites around the world.

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SENSITIVE

Practical Detection Limits (PDL) are as low as 100 cells per sample with a dynamic range over seven orders of magnitude. Low detection limits are particularly important when evaluating whether bioaugmentation is needed or an unnecessary expense.

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SPECIFIC

Target specific bacterial groups (e.g., Dehalococcoides, Dehalobacter, Dehalogenimonas) and functional genes (e.g. vinyl chloride reductases, anaerobic benzene carboxylase) responsible for contaminant biodegradation.

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COST EFECTIVE

Fast turnaround time (7-10 days), with rush service available, so you can make decisions and take action quickly. CENSUS qPCR is inexpensive and ultimately saves money by allowing site managers to make more informed decisions.

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FLEXIBLE

Analysis can be performed on almost any type of sample (water, soil, sediments, Bio-Traps®, and others).

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CUSTOMIZEABLE

Assays are available for quantification of many microorganisms and functional genes responsible for biodegradation of a broad spectrum of contaminants. Custom assays can also be developed to fit your needs.

HOW CENSUS qPCR ER WORKS:

Along with contaminant concentrations and geochemical parameters, the concentrations of specific microorganisms and functional genes responsible for contaminant biodegradation is a key component of remedy selection and performance monitoring at sites impacted by chlorinated solvents, petroleum hydrocarbons, and emerging contaminants.

Use CENSUS qPCR to help answer…

  • How feasible is MNA? Is enhanced bioremediation needed? Is bioaugmentation needed?
    • What are the concentrations of contaminant degraders under existing conditions?
    • Are degrader concentrations greater in impacted wells than background wells?
    • Based on the MI Database, are concentrations of contaminant degraders low, medium, or high in the plume?

  • Is enhanced bioremediation effective?
    • Did concentrations of contaminant degraders and functional genes increase in response to treatment?
    • Are additional types of degraders and functional genes now detected that were previously below detection limits?

RESOURCES

CASE STUDIES

In Situ Microcosms: Screening MNA vs Sulfate Injection at a BTEX Impacted Site

• The ISM study conducted at this site provided clear, actionable evidence that increasing the sulfate concentration at the site would stimulate hydrocarbon biodegradation. Further, this evidence was provided in a significantly more cost effective manner than a bench-scale treatability study or a pilot scale study and in a more timely manner.

CENSUS qPCR: Actionable Data for Evaluating MNA

• A petroleum pipeline release in a remote area had gone undiscovered for a number of years and groundwater was impacted with BTEX. Groundwater monitoring results suggested that BTEX concentrations were decreasing although results were variable.

• Trends in decreasing BTEX concentrations were validated as biological in origin supporting an MNA management strategy and avoiding the greater costs of enhanced remediation.

CENSUS qPCR: Evaluating Biostimulation to Overcome DCE Stall

• A site impacted by PCE and TCE was experiencing an accumulation of cis-DCE (DCE stall). CENSUS qPCR quantification demonstrated that Dehalococcoides concentrations were too low for effective rates of complete reductive dechlorination.

• Avoiding the expense of bioaugmentation resulted in significant cost savings while achieving treatment goals with biostimulation alone.

FIND OUT MORE:
How CENSUS® qPCR Can Help With These Contaminants

QUESTIONS?