Microbial Insights has extensive expertise in environmental microbiology and providing remediation strategies for the agriculture industry. We offer highly accurate, cost-effective, and customizable molecular biological tools (MBTs) to provide the comprehensive data needed to help agri-businesses, consultants, and government agencies make informed and effective agricultural management, environmental assessment, and remediation decisions.

ISSUE:  Production-Plant-Microbe interactions significantly influence plant health and productivity whether through nutrient availability, phytohormones, disease, or disease suppression.  Environmental Remediation-Bioremediation can be a cost effective solution for soil and groundwater impacted by pesticides. 

IMPACT: Crop yields + Disease in plants + Soil and Water Quality

THREATS: Agricultural Output + Environmental Consequences.

PATH TO RESOLUTION: Our superior molecular biological tools and data interpretation provide you with the detailed insight you need to make informed decisions that impact agricultural management and environmental remediation. Genomics and other DNA based methods can cost-effectively answer microbiological questions that are simply not possible with other methods. Our analysis is 100% unbiased. Microbial Insights does not sell biologicals or remediation products.  We provide the MBTs and reporting that help you make the most informed decisions, based on the best possible data and analysis.  

  • Quantify microorganisms responsible for processes in the nitrogen cycle including Nitrogen Fixing Bacteria (NIF), Ammonia Oxidizing Bacteria (AMO), and Denitrifying Bacteria (DNF). 
  • Generate an overall profile of the rhizosphere microbial community and determine if potential nitrogen fixing, phosphate solubilizing, siderophore producing, or other plant growth promoting bacteria are present. 
  • Examine the microbial community for bacteria associated with degradation of common pesticides.

Agriculture INDUSTRY

THE MI ADVANTAGE

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Explore Our Molecular Biological Tools (MBTs) and Site Logic Services

Nutrients and Plant Growth Promoting Rhizobacteria (PGPR) For absolute quantification of microorganisms or functional genes responsible for processes in the nitrogen cycle including nitrogen fixation, nitrification, and denitrification, including Nitrogen Fixing Bacteria (NIF), Ammonia Oxidizing Bacteria (AMO), and Denitrifying Bacteria (DNF).

Nutrients and Plant Growth Promoting Rhizobacteria (PGPR) Generate an overall profile of the rhizosphere microbial community and determine if potential nitrogen fixing, phosphate solubilizing, or siderophore producing bacteria are present.  NGS can also reveal the presence of nitrifying and denitrifying bacteria.  NGS results provide relative abundances of detected genera as % of total reads.

Pesticides Examine the microbial community for bacteria associated with degradation of common pesticides.

Fungal:Bacterial (F:B) Biomass Ratios. This ratio has been linked to overall soil health, carbon cycling, and nutrient usage. In general, higher soil fertility with fewer disturbances results in a higher percentage of fungal biomass. Phospholipid Fatty Acid (PLFA) analysis provides a reliable F:B biomass ratio for soil samples.

RESOURCES

AVAILABLE CENSUS® qPCR ASSAYS RELATED TO THE NITROGEN CYCLE

 

TARGETMI CODERELEVANCE / DATA INTERPRETATION
Ammonia Oxidizing BacteriaAMOThe AMO assay targets the ammonia monooxygenase gene (amoA) that encodes the enzyme responsible for the initial oxidation of ammonia in the nitrification process.
Ammonia Oxidizing ArchaeaAOATaxonomic gene for archaea that can oxidize ammonia to nitrite.
Nitrite Oxidizing BacteriaNOBResponsible for converting nitrite to nitrate.
Nitrite Oxidizing BacteriaNOR/NXRTargets the gene encoding the enzyme responsible for the last step in nitrification.
Denitrifying BacteriaDNFThe assays target the genes encoding two types of nitrite reductase enzymes (nirS and nirK) for quantification of denitrifying bacteria.
Archaeal Nitrate ReducersADNFNIRKFunctional gene responsible for converting nitrite to nitric oxide in archaeal organisms.
Archaeal Nitrate ReducersADNFNIRSFunctional gene responsible for converting nitrite to nitric oxide in archaeal organisms.
Anaerobic Ammonia Oxidizing BacteriaAMXNIRSThis assay targets a nitrite reductase gene involved in converting nitrite to nitric oxide.
Anaerobic Ammonia Oxidizing BacteriaAMXNIRKThis assay targets a nitrite reductase gene involved in converting nitrite to nitric oxide.
Nitrogen Fixing BacteriaNIFTargets the gene nifD related to the conversion of nitrogen to ammonium which can be assimilated by organisms.

EASY STEPS TO ADDRESS YOUR AGRICULTURAL NEEDS

1) Call us at 865.573.8188 or email at info@microbe.com
2) A friendly member of our team will advise you on the right tools for the job
3) Order and receive your supplies
4) Perform sampling according to our clear and easy protocols
5) Fill out the chain of custody form
6) Send to the nearest Microbial Insights location
7) Receive your unbiased report with actionable data and insight

QUESTIONS?

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