CENSUS qPCR is a DNA based molecular microbiological method (MMM) to accurately quantify total bacteria, total archaea, and specific microorganisms like sulfate reducing bacteria (SRB) that are commonly responsible for microbiologically influenced corrosion (MIC) and souring.  Also consider QuantArray®, an advanced qPCR method that can simultaneously quantify a broad spectrum of targets in a single run.

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CENSUS qPCR MIC ADVANTAGES:

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ACCURATE

Since the overwhelming majority (>99%) of microorganisms cannot be grown in artificial media, traditional methods (e.g. MPNs and plate counts) are not accurate and may vastly underestimate MIC threats. With CENSUS®qPCR, DNA is extracted directly from the field sample removing the need to grow the bacteria and eliminating biases associated with plate counts and MPNs.

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QUANTITATIVE

Absolute quantification of the concentrations of total bacteria, total archaea, and specific microbial groups like sulfate reducing bacteria (SRB) to monitor abundances over time or in response to biocides or other MIC mitigation efforts. Results reported as cells/mL, cells/g, etc.

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SENSITIVE

Practical Detection Limits (PDL) are as low as 100 cells per sample with a dynamic range over seven orders of magnitude.

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SPECIFIC

Target specific bacterial groups (e.g., sulfate reducing bacteria, methanogens, iron reducing bacteria) responsible for MIC.

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COST EFFECTIVE

Fast turnaround time (7-10 days), with rush service available, so you can make decisions and take action quickly. CENSUS®qPCR is inexpensive and ultimately saves money by allowing corrosion engineers and operators to make more informed MIC mitigation decisions.

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FLEXIBLE

Analysis can be performed on almost any type of sample (water, solids, corrosion coupons, swabs, pigging solids, scrapings, and others).

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CUSTOMIZABLE

Assays are available for quantification of many different MIC associated microbes. Custom assays can also be developed to fit your needs.

HOW TO USE CENSUS qPCR MIC:

For MIC threat assessment, routine monitoring, and evaluating the effectiveness of biocides or other mitigation activities, CENSUS qPCR quantification of total and specific MIC causing microorganisms provides the actionable data needed to make decisions.

Use CENSUS qPCR to help answer…

  • Is MIC a threat?
  • Are concentrations of total bacteria, SRB, and other MIC associated microorganisms increasing?
  • Was the biocide effective?
  • Did concentrations of MIC associated microorganisms decrease after treatment?
  • Have concentrations of MIC causing microorganisms rebounded?

RESOURCES

AVAILABLE CENSUS qPCR ASSAYS TO HELP WITH MIC, SOURING AND MITIGATION

TARGETMI CODERELEVANCE / DATA INTERPRETATION
Total EubacteriaEBACMIC is initiated by growth of a biofilm on the material surface. Monitoring total bacteria provides a general measure for evaluating bacterial growth in the system.
Total ArchaeaARCArchaea are another general group of single celled microorganisms which, like bacteria, can initiate and contribute to MIC. Depending upon types and environmental conditions, total archaea can outnumber total bacteria and be a more important factor in MIC.
Sulfate Reducing BacteriaAPSSulfate reducing bacteria (SRB) consume hydrogen, produce hydrogen sulfide and are the group of microorganisms most commonly implicated in the pitting corrosion of various metals.
Sulfate Reducing ArchaeaSRASulfate reducing archaea consume hydrogen, produce hydrogen sulfide and have been implicated in MIC at elevated temperatures.
MethanogensMGNMethanogens utilize hydrogen for growth, can contribute to cathodic depolarization and can cause corrosion rates comparable to sulfate reducing bacteria.
Acid Producing BacteriaAGNAcetogenic bacteria are strict anaerobes that produce acetate from the conversion of H2-CO2, CO, or formate. Hydrogen mediated acetogenesis has been demonstrated in high pressure natural gas pipelines confirming the in situ activity of this bacterial group. Further, the presence of acetic acid is known to exacerbate carbon dioxide corrosion of carbon steel.
FermentersFERAnaerobic bacteria produce organic acids and hydrogen. Acid production can lead to localized drops in pH facilitating corrosion while hydrogen production can support growth of other MIC associated organisms including SRB.
Iron Reducing Bacteria (other)IRBIron reducing bacteria reduce insoluble ferric iron to soluble ferrous iron potentially facilitating the removal of protective corrosion products formed on exposed iron alloy surfaces. However, other studies have suggested that the actions of IRB can inhibit corrosion through a variety of mechanisms. This assay targets iron reducing bacteria such as Deferribacter, Ferrimonas, Geopsychrobacter, Geothermobacter, Geothrix, Geovibrio, Geothermobacterium and Albidiferax. Please note that Geobacter and Shewanella are also common iron reducing bacteria which need to be ordered as separate assays.
Iron Reducing Bacteria (Geobacter)GEOIron reducing bacteria reduce insoluble ferric iron to soluble ferrous iron potentially facilitating the removal of protective corrosion products formed on exposed iron alloy surfaces. This assay targets a common iron reducing bacteria, Geobacter.
Iron Reducing Bacteria (Shewanella)SHWAnaerobic bacteria which can utilize cathodic hydrogen as an energy source, reduce ferric iron and sulfite to ferrous iron and sulfide indicating that it can play a role in MIC.
Iron Reducing ArchaeaIRATargets two genera of iron reducing archaea, Ferroglobus and Geoglobus.
Iron Oxidizing BacteriaFeOBIron oxidizing bacteria are a group of microorganisms commonly implicated in metal deposition and tubercle formation.
Manganese Oxidizing BacteriaMnOBLike iron oxidizing bacteria, manganese oxidizing bacteria are capable of making deposits of metal oxides.
Sulfur Oxidizing BacteriaSOBOften aerobic bacteria oxidize sulfide or elemental sulfur producing sulfuric acid. Commonly implicated in the corrosion of concrete.

Nitrate Reducing BacteriaDNFIncreasingly, nitrate addition is being used to stimulate growth of nitrate reducing bacteria as a bioexclusion strategy to combat SRB-mediated reservoir souring and MIC. The DNF assay quantifies target genes encoding enzymes responsible for a key step in biological nitrate reduction.
Archaeal Nitrite Reducing BacteriaADNFSimilar to the DNF assay, ADNF quantifies the two types of nitrite reductase genes (nirS and nirK) found in archaeal organisms.
Ammonia Oxidizing BacteriaAOBAmmonia oxidation or nitrification produces nitric acid causing corrosion of concrete and natural stone. Depending on alkalinity levels, nitrification in water systems can increase lead contamination and increase copper solubility.
Nitrite Oxidizing BacteriaNORTargets the gene encoding the enzyme responsible for the last step in nitrification.
Nitrogen Fixing BacteriaNIFNitrogen fixation converts nitrogen gas into ammonia which can be assimilated by organisms. Nitrogen fixation may become increasingly important in mature biofilms.
Exopolysaccaride ProductionBCEGene involved in the production of exopolysaccharide (EPS) and biofilm formation by some Burkholderia spp.
Deinococcus spp.DCSGenus of bacteria considered very efficient primary biofilm formers and therefore have been implicated in slime formation and biofouling.
Meiothermus spp.MTSLike Deinococcus spp., Meiothermus spp. are efficient primary biofilm formers and frequently implicated in slime formation and biofouling.
Cladosporium spp.CLADThe fungus Cladosporium resinae is such a common fuel contaminant that it has been described as the "kerosene fungus". C. resinae grows on hydrocarbons including alkanes to produce organic acids often linked to the corrosion of aluminum fuel tanks.
Sporomusa spp.

SSPHGenus of anaerobic, acetic acid producing bacteria (homoacetogens). Acetic acid is known to exacerbate carbon dioxide corrosion of carbon steel. Moreover, one Sporomusa species, S. sphaeroides, has been shown to grow with iron as the sole electron donor enhancing corrosion.
Acetic Acid BacteriaAABQuantifies the alcohol dehydrogenase (adhA) genes from acetic acid bacteria (Acetobacter, Gluconobacter, and Komagataeibacter). adhA catalyzes the oxidation of ethanol to acetic acid which can be a potential cause of corrosion.
Glycerol Utilizing BacteriaGLKMicrobial degradation of glycerol, a byproduct of biodiesel production from fats, leads to the generation of VFAs (lactic and propionic acid) both of which have been observed at high concentrations in diesel tanks. VFA production can substantially reduce local pH and also supports the growth of other microbial groups commonly implicated in corrosion. The GLK assay targets a key functional gene in glycerol uptake and utilization.

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