For this project, site managers wanted to know if they could transition to MNA. By utilizing QuantArray®-Petro and SIP, we were able to provide them the analysis to support an MNA management decision.
• During site assessment of a TCE site, compound specific isotope analysis (CSIA) did not demonstrate TCE or cis-DCE degradation, consistent with CENSUS qPCR results indicating low concentrations of Dehalococcoides (DHC).
• Multiple lines of evidence increased stakeholder confidence in full-scale treatment.
– QuantArray®-MIC was performed on swab samples from internally pitted and adjacent non-pitted areas of pipeline components as part of a multiple lines of evidence approach to evaluate microbiologically influenced corrosion (MIC).
– This case study shows how NGS revealed diverse microbial communities and the presence of microbial groups commonly implicated in MIC, including SRB, methanogens and acetogens.
• A homeowner dependent on a domestic water well suddenly saw an accumulation of a black precipitant clogging the pre-filter of their water treatment system. Two years previous to this observation there was a release of gas condensate hydrocarbons and saltwater upgradient of their property.
• The results of the QuantArray®-Petro analysis provided evidence of hydrocarbon impact in the drinking water aquifer upgradient of the homeowner’s property in a remarkably cost-effective manner and contributed to a settlement of this case to the benefit of the homeowner.
• A pilot study was conducted at a site where electron donor was injected into groundwater impacted by a mixture of chlorinated solvents.
• QuantArray®-Chlor and NGS provided complimentary evidence of both biotic and abiotic reductive dechlorination in response to the injection of the electron donor giving the client confidence in this remediation strategy expanding electron donor injection to the entire site.
• The ISM study conducted at this site provided clear, actionable evidence that increasing the dissolved oxygen concentration at the site would stimulate hydrocarbon biodegradation. Further, this evidence was provided in a significantly more cost effective manner than a bench-scale treatability study or a pilot scale study and in a more timely manner.
• The ISM study conducted at this site provided clear, actionable evidence that increasing the sulfate concentration at the site would stimulate hydrocarbon biodegradation. Further, this evidence was provided in a significantly more cost effective manner than a bench-scale treatability study or a pilot scale study and in a more timely manner.
• At a petroleum pipeline release in a remote area Bio-Traps® and CENSUS® qPCR for BTEX catabolic functional genes proved stimulation of BTEX biodegradation in impacted groundwater and provided an additional line of evidence to support acceptance of an MNA management strategy at the site.
• Contaminant trends, geochemistry, CENSUS® qPCR, and NGS provided multiple lines of evidence supporting an MNA management strategy and avoiding the greater costs of enhanced remediation.
• Site managers were considering monitored natural attenuation (MNA), biostimulation, and bioaugmentation as remediation strategies at a site impacted by a complex mixture of chlorinated hydrocarbons including PCE, TCE, 1,1,2-TCA, 1,2-DCA, cis-DCE, and VC along with high concentrations of sulfate.
• QuantArray®-Chlor analysis provided actionable data for remedy selection allowing timely decisions and significant cost savings in site management.