Use CENSUS qPCR or QuantArray®-Chlor to quantify Dehalococcoides and functional genes responsible for reductive dechlorination of PCE, TCE, DCE, vinyl chloride and other chlorinated compounds.
Dehalococcoides were the first bacterial group isolated and proven to be capable of complete reductive dechlorination of PCE and TCE to ethene. Since then, CENSUS qPCR and QuantArray-Chlor quantification of Dehalococcoides and reductive dehalogenase genes (TCE, BVC, and VCR) have become an indispensable component of assessment, remedy selection, and performance monitoring at chlorinated solvent sites. In fact, Lu et al. (2006) have proposed using a Dehalococcoides concentration of 1.0E+04 cells/mL as a screening criterion to identify sites where biological reductive dechlorination will proceed at “generally useful” rates.