Microbial Data to Support Landfarming and Biopiles

Landfarming and biopiles are two ex situ methods for soil and sediment remediation. Landfarming relies on agricultural practices, including tilling, fertilizing, and aerating, to stimulate contaminant biodegradation. Excavated soil or dredged sediment is spread over a prepared area and potentially mixed with the soil underneath. The addition of nutrients, oxygen, and moisture create an environment favorable for aerobic biodegradation. A bulking agent like hay may also be added to increase soil permeability and air circulation. Proper controls must be in place to prevent spreading contamination off-site through leachate, vapor, or dust.

Bioremediation via biopiles is like landfarming except the excavated soil or sediment is placed in piles or mounds on an impermeable surface. A passive or forced air aeration system may also be in place. The biodegradation processes produce heat, which raises the soil temperature and stimulates further biodegradation. Biopiles may be covered to retain heat and prevent precipitation from creating excessive leachate.

Both methods are primarily used to treat compounds that degrade aerobically, including fuel hydrocarbons like those found at underground storage tank (UST) sites, drill cuttings, and petroleum refinery wastes. The duration of the treatment depends on the mixture of contaminants as low-molecular weight compounds are more readily degraded compared to compounds like naphthalene and other PAHs that have higher molecular weights. Microbial analysis can be used to monitor hydrocarbon-degrading microorganisms over the course of treatment.

Molecular Biological Tools for Landfarming and biopiles

MBTs to Assess Microbial Degraders in Soil and Sediment

MBTs, such as CENSUS® qPCR and QuantArray®-Petro, are routinely employed to quantify concentrations of microorganisms and functional genes responsible for degrading the petroleum hydrocarbons (including BTEX, naphthalene, and alkanes). For sites undergoing bioremediation, compare baseline microbial results to those from different timepoints during the treatment to determine if concentrations of contaminant-degrading microorganisms are increasing.

Frequently asked questions

Can Microbial Insights receive soil samples from international locations?

Yes, Microbial Insights has an international soil permit and has received samples without issue from many different countries. Please see the international shipping page for additional information.

How much sample do I need to collect?

Almost any type of sample matrix can be analyzed using MBTs. Soil or sediment samples are recommended for landfarming and biopile projects. Approximately 50 g of sample should be collected for each location. Complete sampling protocols can be found here.

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BIOREMEDIATION Resources

EASY STEPS TO SUPPORT LANDFARMING AND BIOPILES

1) Call us at 865.573.8188 or email at [email protected]
2) A friendly member of our team will advise you on the right tools for the job
3) Order and receive your supplies
4) Perform sampling according to our clear and easy protocols
5) Fill out the chain of custody form
6) Send to the nearest Microbial Insights location
7) Receive your unbiased report with actionable data and insight

Have a different corrective action plan you are considering?  Click the button information for other plan types and how Microbial Insights can help your project.