Microbial Data to Support Monitored Natural Attenuation

When remediation by engineered methods is not practical (e.g., large dilute plumes or impacts located far from the source area), monitored natural attenuation (MNA) may be the most promising strategy to achieve remedial objectives. MNA relies on naturally occurring physical processes (dispersion, volatilization, and dilution), abiotic degradation, and biodegradation to reduce contaminant concentrations and risks over time. To evaluate the feasibility and performance of MNA as a site management strategy, a multiple-lines-of-evidence approach is recommended, collecting:

  • Chemistry to demonstrate that contaminant concentrations are decreasing or stable with a statistical approach like the Mann-Kendall test.
  • Geochemistry to compare electron acceptor concentrations (e.g., dissolved oxygen, nitrate, iron, sulfate, methane) as an indirect indicator of microbial activity.
  • Microbiology to directly evaluate biodegradation as a component of MNA using molecular biological tools (MBTs).

Molecular Biological Tools for MNA

MBTs to Assess MNA at Petroleum Sites

MBTs, such as CENSUS® qPCR, QuantArray®-Petro, andQuantArray®-NSZD, are routinely employed to quantify concentrations of microorganisms and functional genes responsible for degrading the petroleum hydrocarbons (including BTEX, naphthalene, and alkanes) to assess a site’s biodegradative potential for supporting MNA.

Complementing these MBTs, Stable Isotope Probing (SIP) is used to conclusively demonstrate the biodegradation of a specific contaminant (e.g., benzene or naphthalene). The method uses a Bio-Trap® sampler amended with a specially synthesized 13C form of the contaminant of concern and deployed in an impacted monitoring well. After retrieval of the Bio-Trap®, the presence of 13C-labeled biodegradative end products provides conclusive evidence that the contaminant was biodegraded, providing a strong line of evidence for MNA as an effective remediation strategy.

MBTs to Assess MNA at Chlorinated Solvent Sites

Aerobic cometabolism of TCE and other chlorinated solvents can be an important component of MNA. However, this degradative mechanism can be difficult to detect in the chemical data because it does not produce measurable daughter products, unlike anaerobic reductive dechlorination. Microbial data from CENSUS® qPCR or QuantArray®-Chlor analyses can help determine if aerobic cometabolism is a meaningful contributor to MNA by quantifying microorganisms and functional genes responsible for co-oxidizing chlorinated hydrocarbons.

Chlorinated compounds are also susceptible to abiotic degradation catalyzed by common iron-bearing minerals. A variety of chlorinated solvents (including PCE, TCE, cis-DCE, and vinyl chloride) react with the mixed-valence iron mineral magnetite, which is the most abundant magnetic mineral in natural sediments. Other reactive iron minerals, including mackinawite and pyrite, may also be present in naturally reducing anoxic aquifers. Soil or Min-Trap® samples can be analyzed by magnetic susceptibility and x-ray diffraction (XRD) to detect these minerals and assess the potential for abiotic degradation.

Frequently asked questions

Is there a method to evaluate the feasibility of multiple treatment options before implementing a full-scale remedy?

Consider an In Situ Microcosm (ISM) study to compare MNA to enhanced bioremediation options like biostimulation and bioaugmentation under field conditions.

Is there a correlation between qPCR results for aerobic oxidation genes and TCE degradation rates?

Yes, Wilson et al. (2019) found that qPCR results for several oxygenase genes correlated with first order TCE degradation rates, concluding that concentrations of these genes as measured by CENSUS® qPCR or QuantArray®-Chlor can be used as a screening tool to determine if MNA is a feasible remedy for aerobic TCE sites.

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Monitored natural Attenuation Case Studies

QuantArray®-Petro: Evaluating a Transition to MNA

For this project, site managers wanted to know if they could transition to MNA. By utilizing QuantArray®-Petro and SIP, we were able to provide them the analysis to support an MNA management decision.

SIP: Evaluating MNA at a Manufactured Gas Plant

– Site stakeholders needed conclusive evidence that biodegradation of benzene and naphthalene was occurring under existing site conditions to accept MNA instead of a more aggressive strategy.

– Stable isotope probing (SIP) studies using Bio-Traps® amended with 13C benzene or 13C naphthalene conclusively demonstrated in situ biodegradation occurred under existing site conditions.

QuantArray®-Petro: MNA Assessment at a Crude Oil Impacted Site

At a crude oil impacted site, project managers were considering MNA as a site management strategy based on favorable contaminant concentration trends and geochemistry in groundwater. However, additional lines of evidence were required.

Together with contaminant concentration trends and geochemistry, the direct microbial data provided multiple lines of evidence to support a confident MNA decision resulting in significant cost savings over enhanced remediation.

CENSUS® qPCR: Actionable Data for Evaluating MNA

• A petroleum pipeline release in a remote area had gone undiscovered for a number of years and groundwater was impacted with BTEX. Groundwater monitoring results suggested that BTEX concentrations were decreasing although results were variable.

• Trends in decreasing BTEX concentrations were validated as biological in origin supporting an MNA management strategy and avoiding the greater costs of enhanced remediation.

EASY STEPS TO SUPPORT MONITORED NATURAL ATTENUATION

1) Call us at 865.573.8188 or email at [email protected]
2) A friendly member of our team will advise you on the right tools for the job
3) Order and receive your supplies
4) Perform sampling according to our clear and easy protocols
5) Fill out the chain of custody form
6) Send to the nearest Microbial Insights location
7) Receive your unbiased report with actionable data and insight

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