QuantArray® MIC, an advanced qPCR method, rapidly detects and quantifies key microorganisms and functional genes responsible for microbiologically influenced corrosion (MIC) and oilfield souring in a single analysis.
QuantArray® MIC ADVANTAGES:
Direct analysis of sample DNA removes the need to grow the bacteria thus eliminating biases associated with traditional approaches (e.g. plate counts and BARTs).
Absolute quantification of concentrations of total bacteria, total archaea, and specific microbial groups to monitor abundances over time or in response to treatment. Results reported as cells/mL, cells/g, etc.
Practical Detection Limits (PDL) are as low as 100 cells per sample with a dynamic range over seven orders of magnitude.
Target specific bacterial groups (e.g. sulfate reducing bacteria, methanogens) associated with MIC.
In a single analysis, quantify total bacteria, total archaea, and a broad spectrum of common MIC microorganisms (e.g. SRB, SRA, methanogens, IRB, FeOB, etc.) Same accuracy and turnaround time as CENSUS®qPCR but more comprehensive and even more cost-effective.
Assays are available for quantification of MIC associated microbes for which traditional assays are not available, and custom assays can be developed to fit your needs.
QuantArray® MIC analysis can be performed on almost any type of sample (water, solids, corrosion coupons, swabs, pigging debris, scrapings, Bio-Traps®, and others).
ALL ORGANISM TARGETS LISTED BELOW ARE INCLUDED IN A SINGLE QUANTARRAY® qPCR ANALYSIS
Total Bacteria (EBAC)
Total Archaea (ARC)
Sulfate Reducing Bacteria (APS)
Sulfate Reducing Archaea (SRA)
MIC Hydrogenase (MicH)
TatC Translocase (TatC)
Iron Reducing Bacteria – Other (IRB)
IRB Geobacter (IRG)
IRB Shewanella (IRS)
Iron Reducing Archaea (IRA)
Iron Oxidizers (FeOB)
Manganese Oxidizing Bacteria (MnOB)
Sulfur Oxidizing Bacteria (SOB)
Denitrifying Bacteria (nirK)
Denitrifying Bacteria (nirS)
Ammonia Oxidizing Bacteria (AMO)
Nitrite Oxidizing Bacteria (NOR)
Nitrogen Fixers (NIF)
Burkholderia cepacian Exopolysaccharide (BCE)
Deinococcus spp. (DCS)
Meiothermus spp. (MTS)
HOW TO USE QuantArray® MIC:
QuantArray® MIC provides simultaneous quantification of key organisms and functional genes involved in MIC, as well as oilfield souring, proving a more comprehensive assessment. Evaluation of the microorganisms present within a system permits managers to:
- Quantify MIC bacteria and accurately assess corrosion potential
- Evaluate the effectiveness of treatments and O&M measures
All of the organisms listed above are included in a single QuantArray® qPCR analysis.