This Top Tips bulletin explains how stable isotope probing (SIP) provides conclusive, in situ evidence of biodegradation for petroleum hydrocarbons, fuel oxygenates, and selected emerging contaminants. It highlights best practices for selecting target compounds, interpreting results, and integrating SIP with monitored natural attenuation (MNA) or in situ microcosm studies to support defensible site decisions.
This Top Tips bulletin explains how monitored natural attenuation (MNA) can be effectively evaluated and supported at petroleum‑impacted sites using multiple lines of evidence. It highlights how molecular biological tools such as QuantArray®‑Petro, CENSUS® qPCR, stable isotope probing, and in situ microcosms provide direct, defensible proof of biodegradation and help compare MNA with enhanced remediation…
This white paper describes how stable isotope probing (SIP), QuantArray®‑Petro, and QuantArray®‑NSZD work together to evaluate biodegradation of petroleum hydrocarbons under real site conditions. By combining proof of degradation with pathway‑specific and microbial community data, these complementary tools support defensible decisions for monitored natural attenuation (MNA), bioremediation, and long‑term site management.
For more than 20 years, In Situ Microcosms (ISMs), have been used as an in‑well groundwater remediation tool that enables direct, side‑by‑side comparison of monitored natural attenuation (MNA), biostimulation, and bioaugmentation under actual aquifer conditions. ISMs integrate contaminant chemistry, geochemistry, and molecular microbiology to provide defensible, EPA‑relevant lines of evidence for remediation technology selection and…
This technical bulletin shows how molecular biological tools can be integrated throughout the stages of remediation (site characterization, remedy selection, performance monitoring, and closure) to improve confidence in bioremediation decisions. It highlights the use of qPCR, QuantArray®, in situ microcosms, and stable isotope probing to reduce uncertainty, optimize remedies, and support transition to monitored natural…
This technical bulletin explains how stable isotope probing (SIP) provides conclusive, in situ proof of contaminant biodegradation by tracing ¹³C‑labeled compounds into microbial biomass and carbon dioxide. It demonstrates how SIP supports remedy selection, performance monitoring, and monitored natural attenuation (MNA) decisions using real‑world case studies and Bio‑Trap® deployments.
This technical bulletin provides guidance on designing and evaluating electron acceptor addition to enhance bioremediation of petroleum hydrocarbons when monitored natural attenuation is insufficient. It explains how tools such as CENSUS® qPCR, QuantArray®‑Petro, Bio‑Traps®, and in situ microcosms help select the appropriate electron acceptor, optimize dosing, and verify treatment performance.
This technical bulletin describes how integrating chemical, geochemical, and microbial data provides a robust multiple lines of evidence (MLOE) framework for evaluating bioremediation, monitored natural attenuation (MNA), and enhanced treatment performance. It highlights the use of molecular biological tools, isotope techniques, sequencing, and in situ microcosms to reduce uncertainty, improve remedy selection, and support defensible…
Bio-Traps® are innovative passive samplers that reveal which microbes are truly active and driving biodegradation at contaminated sites. This paper explains how Bio-Traps® help practitioners confidently evaluate natural attenuation, assess remediation performance, and make better site-management decisions.
This product brochure provides an overview of Bio-Traps®, which are passive microbial samplers that collect microbes over time for a better understanding of biodegradation potential. Bio-Traps® can be analyzed using a variety of molecular biological tools, including CENSUS® qPCR, QuantArray®, and Next Generation Sequencing.