This white paper provides insight into microorganisms and enzymes associated with 1,4-dioxane biodegradation. Compared to advanced oxidation processes, bioremediation strategies are a more cost-effective and sustainable alternative for 1,4-dioxane removal. Molecular biological tools and passive samplers for monitoring microorganisms capable of degrading 1,4-dioxane include qPCR, stable isotope probing, in situ microcosm testing, carbon-14 assay analysis,…
This handout describes how microbial monitoring at sites impacted with 1,4-dioxane can help identify biodegradation potential, support remediation design, and improve regulatory compliance and risk assessment. Microbial analyses that support 1,4-dioxane bioremediation projects include CENSUS® qPCR, stable isotope probing, and whole genome sequencing.
A site impacted by chlorobenzene was managed by MNA. Site managers asked: “Can we confirm that observed decreases in chlorobenzene concentrations are resulting from biodegradation as opposed to dilution?” and “Would injection of an oxygen release compound result in enhanced rates of aerobic biodegradation of chlorobenzene under site conditions?” The answers to both questions were yes. …
Site stakeholders needed conclusive evidence that biodegradation of benzene and naphthalene was occurring under existing site conditions to accept MNA instead of a more aggressive strategy. Stable isotope probing (SIP) studies using Bio-Traps® amended with 13C benzene or 13C naphthalene conclusively demonstrated in situ biodegradation occurred under existing site conditions. MNA was accepted as the remediation strategy.
Mass reduction by DPE and SVE at a hydrocarbon- and MTBE-impacted site had become uneconomical. Site managers asked: “Can we transition to MNA?” The answer, for the most part, was yes. Post SVE, hydrocarbon and MTBE concentrations showed decreasing trends in most monitoring wells. Additional strong lines of evidence for in situ biodegradation of benzene and MTBE under…
This article offers an overview of how to leverage molecular biological tools to evaluate and enhance petroleum hydrocarbon remediation. It provides practical guidance and field case studies for a variety of microbial analyses, including quantitative polymerase chain reaction (qPCR), qPCR arrays, RT-qPCR, stable isotope probing (SIP), targeted taxonomic sequencing, and metagenome sequencing.
This open access book synthesizes important advances in the assessment and management of soil and groundwater systems contaminated with petroleum hydrocarbons. Chapter 10: Molecular Biological Tools Used in Assessment and Remediation of Petroleum Hydrocarbons in Soil and Groundwater is co-authored by Dora M. Taggart and Trent A. Key.
This brochure provides guidance on selecting appropriate molecular biological tools, including QuantArray®, CENSUS® qPCR, SIP, the abiotic panel, and in situ microcosms, based on the chlorinated compounds present and the chosen remedial approach.
This brochure provides guidance on selecting appropriate molecular biological tools, including QuantArray®, CENSUS® qPCR, SIP, and in situ microcosms, based on the petroleum hydrocarbon contaminants present and the chosen remedial approach.
This product brochure provides an overview of Stable Isotope Probing (SIP), which is an innovative method that tracks the environmental fate of a 13C-labeled contaminant of concern to unambiguously demonstrate biodegradation under aquifer conditions.