Lessons learned from 20 years of MBTs in petroleum hydrocarbon remediation

Summary:

This article offers an overview of how to leverage molecular biological tools to evaluate and enhance petroleum hydrocarbon remediation. It provides practical guidance and field case studies for a variety of microbial analyses, including quantitative polymerase chain reaction (qPCR), qPCR arrays, RT-qPCR, stable isotope probing (SIP), targeted taxonomic sequencing, and metagenome sequencing.