This article offers an overview of how to leverage molecular biological tools to evaluate and enhance petroleum hydrocarbon remediation. It provides practical guidance and field case studies for a variety of microbial analyses, including quantitative polymerase chain reaction (qPCR), qPCR arrays, RT-qPCR, stable isotope probing (SIP), targeted taxonomic sequencing, and metagenome sequencing.
This article outlines procedures and considerations for utilizing Bio-Extract™ in-field DNA isolation kits and field-portable qPCR with the FieldQuant™ platform. Comparison data and replicate results are also included, demonstrating the sound science of this innovative system.
This article discusses considerations for designing Min-Trap® studies as well as the necessary procedures for incorporating these passive mineral samplers at a site. From capturing in situ reactive mineral formation for the evaluation of remediation strategies, to understanding PFAS contaminant distribution in the subsurface, the Min-Trap® approach has broad applicability, is repeatable, and avoids expensive…
This article provides an important link between qPCR data for the benzylsuccinate synthase gene (bssA) and estimated rate constants for anaerobic toluene biodegradation.
This open access book synthesizes important advances in the assessment and management of soil and groundwater systems contaminated with petroleum hydrocarbons. Chapter 10: Molecular Biological Tools Used in Assessment and Remediation of Petroleum Hydrocarbons in Soil and Groundwater is co-authored by Dora M. Taggart and Trent A. Key.