This paper provides an overview of microorganisms involved in bioscale production and dissolution, which can cause issues in a variety of industries, including oil and gas, mining, and industrial heating systems. It also highlights how molecular biological tools such as qPCR (CENSUS®), next generation sequencing and whole genome sequencing enable effective monitoring and mitigation of…
A site impacted by PCE and TCE was experiencing an accumulation of cis-DCE (DCE stall). CENSUS qPCR quantification demonstrated that Dehalococcoides concentrations were too low for effective rates of complete reductive dechlorination. Site managers asked: “Is bioaugmentation required or will biostimulation alone be sufficient to result in complete reductive dechlorination?” Monitoring with Bio-Traps® and CENSUS® analysis showed that stimulation…
A petroleum pipeline release in a remote area had gone undiscovered for a number of years and groundwater was impacted with BTEX. Groundwater monitoring results suggested that BTEX concentrations were decreasing although results were variable. The site managers asked for an additional line of evidence of BTEX biodegradation to support acceptance of an MNA management…
At a site impacted by trichloroethene (TCE), biostimulation and bioaugmentation were considered as remediation strategies. The site managers asked: “Is bioaugmentation required at this site for meaningful rates of reductive dechlorination?” The answer was no. An In Situ Microcosm (ISM) study showed conclusively that Dehalococcoides (DHC) was present at the site at low levels but could be significantly stimulated by…
At a petroleum pipeline release in a remote area Bio-Traps® and CENSUS® qPCR for BTEX catabolic functional genes proved stimulation of BTEX biodegradation in impacted groundwater and provided an additional line of evidence to support acceptance of an MNA management strategy at the site. Site managers asked: “Can molecular biological tools provide even more evidence…
An In Situ Microcosm study was conducted to evaluate MNA vs. enhanced anaerobic bioremediation via sulfate reduction at a petroleum impacted site. Site managers asked: “Will the injection of sulfate stimulate anaerobic BTEX biodegradation at this site?” The answer was yes. The ISM study showed conclusively that injection of sulfate at the site would increase the growth of…
This brochure provides an overview of the molecular microbiological methods (MMMs) relevant to addressing microbiologically influenced corrosion (MIC) in the context of midstream oil and gas operations. Relevant applications for CENSUS® qPCR, QuantArray®-MIC and next generation sequencing are discussed.
This brochure provides a list of CENSUS® qPCR assays related to processes in the nitrogen cycle, including nitrification, denitrification, nitrogen fixation, and anaerobic ammonia oxidation.
This article offers an overview of how to leverage molecular biological tools to evaluate and enhance petroleum hydrocarbon remediation. It provides practical guidance and field case studies for a variety of microbial analyses, including quantitative polymerase chain reaction (qPCR), qPCR arrays, RT-qPCR, stable isotope probing (SIP), targeted taxonomic sequencing, and metagenome sequencing.
This article provides an important link between qPCR data for the benzylsuccinate synthase gene (bssA) and estimated rate constants for anaerobic toluene biodegradation.