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Microbially Induced Carbonate Precipitation White Paper

This white paper details mechanisms, emerging applications, and molecular tools for monitoring microbially induced carbonate precipitation (MICP) systems. MICP is a naturally occurring biomineralization process in which microbial activity changes local geochemistry and promotes calcium carbonate precipitation. This process can transform biological activity into measurable changes in mineral formation, contaminant mobility, permeability, and material strength.…

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Microbial Performance KPI Framework

This document provides a cross-industry framework connecting microbial measurements with remediation performance, mine restoration, bioleaching culture quality, asset integrity, agricultural productivity, water quality, and wastewater treatment. Each KPI is structured around a defined measurement, target, performance scale, and key decision question. The framework is designed to help clients translate microbial evidence into clearer operational and…

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Accelerate Military Site Remediation with Molecular Data

Microbial Insights’ molecular biological tools (MBTs) and sampling technologies are used to support site assessment and remediation at military-, munitions-, and aviation-related sites across the United States and beyond. QuantArray®, CENSUS® qPCR, and Min-TrapsTM  can be used to evaluate chlorinated solvents, PFAS, fuels, explosives, or mixed contaminant impact at Department of Defense sites.

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1,4-Dioxane White Paper

This white paper provides insight into microorganisms and enzymes associated with 1,4-dioxane biodegradation. Compared to advanced oxidation processes, bioremediation strategies are a more cost-effective and sustainable alternative for 1,4-dioxane removal. Molecular biological tools and passive samplers for monitoring microorganisms capable of degrading 1,4-dioxane include qPCR, stable isotope probing, in situ microcosm testing, carbon-14 assay analysis,…

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1,4-Dioxane Solutions

This handout describes how microbial monitoring at sites impacted with 1,4-dioxane can help identify biodegradation potential, support remediation design, and improve regulatory compliance and risk assessment. Microbial analyses that support 1,4-dioxane bioremediation projects include CENSUS® qPCR, stable isotope probing, and whole genome sequencing.

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CENSUS® qPCR: Evaluating Biostimulation to Overcome DCE Stall

A site impacted by PCE and TCE was experiencing an accumulation of cis-DCE (DCE stall). CENSUS qPCR quantification demonstrated that Dehalococcoides concentrations were too low for effective rates of complete reductive dechlorination. Site managers asked: “Is bioaugmentation required or will biostimulation alone be sufficient to result in complete reductive dechlorination?” Monitoring with Bio-Traps® and CENSUS® analysis showed that stimulation…

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CENSUS® qPCR: Actionable Data for Evaluating MNA

A petroleum pipeline release in a remote area had gone undiscovered for a number of years and groundwater was impacted with BTEX. Groundwater monitoring results suggested that BTEX concentrations were decreasing although results were variable. The site managers asked for an additional line of evidence of BTEX biodegradation to support acceptance of an MNA management…

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ISMs: Cost Effective Remedy Selection at a TCE Impacted Site

At a site impacted by trichloroethene (TCE), biostimulation and bioaugmentation were considered as remediation strategies. The site managers asked: “Is bioaugmentation required at this site for meaningful rates of reductive dechlorination?” The answer was no.  An In Situ Microcosm (ISM) study showed conclusively that Dehalococcoides (DHC) was present at the site at low levels but could be significantly stimulated by…

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QuantArray®-Chlor: Actionable Data for Performance Monitoring

Electron donor injection was performed at a former chemical manufacturing facility impacted by a complex mixture of chlorinated compounds including PCE, TCE, 1,1,2-TCA, 1,2-DCA, cis-DCE, and VC along with high concentrations of sulfate. Site managers required a comprehensive tool to monitor the performance of the electron donor injection at this complex site to ensure complete…

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QuantArray®-Petro: MNA Assessment at a Crude Oil Impacted Site

At a crude oil impacted site, project managers were considering MNA as a site management strategy based on favorable contaminant concentration trends and geochemistry in groundwater. However, additional lines of evidence were required. Site managers asked: “Is there direct microbial evidence to support BTEX and naphthalene biodegradation at the site?” The answer was yes. Using…