For over a decade, QuantArray®‑Petro, has provided engineers a high‑throughput qPCR platform that simultaneously quantifies functional genes involved in aerobic and anaerobic biodegradation of petroleum hydrocarbons, including BTEX, PAHs, MTBE, and alkanes. QuantArray®‑Petro provides defensible microbial lines of evidence to support monitored natural attenuation (MNA), evaluate enhanced bioremediation, and guide petroleum‑impacted site management decisions.
Used by practitioners around the world since 2012, this paper describes QuantArray®‑Chlor, a high‑throughput qPCR platform that simultaneously quantifies key microorganisms and functional genes involved in the biodegradation of chlorinated solvents. QuantArray®‑Chlor provides comprehensive, actionable data to support site assessment, remedy selection, and performance monitoring for chlorinated groundwater remediation.
This white paper explains how next‑generation sequencing (NGS) is used to characterize subsurface microbial communities and assess biodegradation processes at contaminated groundwater sites. By integrating NGS with qPCR, the approach provides powerful lines of evidence to support monitored natural attenuation (MNA), evaluate remediation performance, and inform data‑driven site management decisions.
For more than 20 years, In Situ Microcosms (ISMs), have been used as an in‑well groundwater remediation tool that enables direct, side‑by‑side comparison of monitored natural attenuation (MNA), biostimulation, and bioaugmentation under actual aquifer conditions. ISMs integrate contaminant chemistry, geochemistry, and molecular microbiology to provide defensible, EPA‑relevant lines of evidence for remediation technology selection and…
This technical bulletin explains how aerobic cometabolism and abiotic degradation can be critical mechanisms supporting monitored natural attenuation (MNA) at chlorinated ethene sites, particularly in large or dilute plumes. It describes how molecular biological tools, mineralogical analyses, and degradation rate studies provide defensible evidence of contaminant destruction beyond traditional groundwater trend analysis.
This technical bulletin shows how molecular biological tools can be integrated throughout the stages of remediation (site characterization, remedy selection, performance monitoring, and closure) to improve confidence in bioremediation decisions. It highlights the use of qPCR, QuantArray®, in situ microcosms, and stable isotope probing to reduce uncertainty, optimize remedies, and support transition to monitored natural…
This technical bulletin explains how stable isotope probing (SIP) provides conclusive, in situ proof of contaminant biodegradation by tracing ¹³C‑labeled compounds into microbial biomass and carbon dioxide. It demonstrates how SIP supports remedy selection, performance monitoring, and monitored natural attenuation (MNA) decisions using real‑world case studies and Bio‑Trap® deployments.
This technical bulletin provides guidance on designing and evaluating electron acceptor addition to enhance bioremediation of petroleum hydrocarbons when monitored natural attenuation is insufficient. It explains how tools such as CENSUS® qPCR, QuantArray®‑Petro, Bio‑Traps®, and in situ microcosms help select the appropriate electron acceptor, optimize dosing, and verify treatment performance.
This technical bulletin describes how integrating chemical, geochemical, and microbial data provides a robust multiple lines of evidence (MLOE) framework for evaluating bioremediation, monitored natural attenuation (MNA), and enhanced treatment performance. It highlights the use of molecular biological tools, isotope techniques, sequencing, and in situ microcosms to reduce uncertainty, improve remedy selection, and support defensible…
This technical bulletin explains how molecular biological tools and advanced samplers enhance the design, evaluation, and performance monitoring of in situ chemical oxidation (ISCO) and in situ chemical reduction (ISCR). It demonstrates how integrating CENSUS® qPCR, QuantArray® suites, and Min‑Trap® samplers provides multiple lines of evidence to link abiotic treatment with bioremediation and long‑term site…