An In Situ Microcosm study was conducted to evaluate MNA vs. enhanced anaerobic bioremediation via sulfate reduction at a petroleum impacted site. Site managers asked: “Will the injection of sulfate stimulate anaerobic BTEX biodegradation at this site?” The answer was yes. The ISM study showed conclusively that injection of sulfate at the site would increase the growth of…
A pilot study was conducted at a site where electron donor was injected into groundwater impacted by a mixture of chlorinated solvents. Site managers asked two questions: “Did the electron donor injection stimulate reductive dechlorination?” and “Did injection of the electron donor also increase the potential for abiotic dechlorination through the generation of biogenic FeS?”…
A homeowner dependent on a domestic water well suddenly saw an accumulation of a black precipitant clogging the pre-filter of their water treatment system. Two years previous to this observation there was a release of gas condensate hydrocarbons and saltwater upgradient of their property. The homeowner and their attorneys asked “Is this black precipitant evidence…
Mass reduction by DPE and SVE at a hydrocarbon- and MTBE-impacted site had become uneconomical. Site managers asked: “Can we transition to MNA?” The answer, for the most part, was yes. Post SVE, hydrocarbon and MTBE concentrations showed decreasing trends in most monitoring wells. Additional strong lines of evidence for in situ biodegradation of benzene and MTBE under…
This brochure provides guidance on how to choose the right sequencing approach for a project. The differences and benefits between next generation sequencing (NGS) and whole genome sequencing (WGS) are discussed.
This brochure provides an overview of whole genome sequencing (WGS), also known as shotgun metagenomics, from the basic workflow to the significance of the data produced. WGS is untargeted, qualitative analysis offering species-level resolution and direct detection of functional genes.
This brochure provides a list of CENSUS® qPCR assays related to processes in the nitrogen cycle, including nitrification, denitrification, nitrogen fixation, and anaerobic ammonia oxidation.
This product brochure provides an overview of the Min-Trap® passive mineral sampler which collects direct mineralogical data without the need for drilling or coring. Min-Traps® are broadly applicable to remediation approaches that rely on the formation, dissolution, and/or transformation of solid-phase minerals to sequester or degrade contaminants.
This article offers an overview of how to leverage molecular biological tools to evaluate and enhance petroleum hydrocarbon remediation. It provides practical guidance and field case studies for a variety of microbial analyses, including quantitative polymerase chain reaction (qPCR), qPCR arrays, RT-qPCR, stable isotope probing (SIP), targeted taxonomic sequencing, and metagenome sequencing.
This article outlines procedures and considerations for utilizing Bio-Extract™ in-field DNA isolation kits and field-portable qPCR with the FieldQuant™ platform. Comparison data and replicate results are also included, demonstrating the sound science of this innovative system.