An In Situ Microcosm study was conducted to evaluate MNA vs. enhanced aerobic bioremediation at a petroleum impacted site. Site managers asked: “Will the injection of an oxygen releasing compound stimulate BTEX biodegradation at this site?” The answer was yes. The ISM study showed conclusively that injection of the an oxygen releasing compound at the site will increase…
A pilot study was conducted at a site where electron donor was injected into groundwater impacted by a mixture of chlorinated solvents. Site managers asked two questions: “Did the electron donor injection stimulate reductive dechlorination?” and “Did injection of the electron donor also increase the potential for abiotic dechlorination through the generation of biogenic FeS?”…
A homeowner dependent on a domestic water well suddenly saw an accumulation of a black precipitant clogging the pre-filter of their water treatment system. Two years previous to this observation there was a release of gas condensate hydrocarbons and saltwater upgradient of their property. The homeowner and their attorneys asked “Is this black precipitant evidence…
QuantArray®-MIC was performed on swab samples from internally pitted and adjacent non-pitted areas of pipeline components as part of a multiple lines of evidence approach to evaluate microbiologically influenced corrosion (MIC). Concentrations of Total Bacteria, Total Archaea, sulfate reducing bacteria (SRB), methanogens, and other microorganisms commonly implicated in MIC were orders of magnitude greater in the…
Mass reduction by DPE and SVE at a hydrocarbon- and MTBE-impacted site had become uneconomical. Site managers asked: “Can we transition to MNA?” The answer, for the most part, was yes. Post SVE, hydrocarbon and MTBE concentrations showed decreasing trends in most monitoring wells. Additional strong lines of evidence for in situ biodegradation of benzene and MTBE under…
This brochure provides an overview of the molecular microbiological methods (MMMs) relevant to addressing microbiologically influenced corrosion (MIC) in the context of midstream oil and gas operations. Relevant applications for CENSUS® qPCR, QuantArray®-MIC and next generation sequencing are discussed.
This article offers an overview of how to leverage molecular biological tools to evaluate and enhance petroleum hydrocarbon remediation. It provides practical guidance and field case studies for a variety of microbial analyses, including quantitative polymerase chain reaction (qPCR), qPCR arrays, RT-qPCR, stable isotope probing (SIP), targeted taxonomic sequencing, and metagenome sequencing.
This open access book synthesizes important advances in the assessment and management of soil and groundwater systems contaminated with petroleum hydrocarbons. Chapter 10: Molecular Biological Tools Used in Assessment and Remediation of Petroleum Hydrocarbons in Soil and Groundwater is co-authored by Dora M. Taggart and Trent A. Key.
This brochure examines how MBTs can be leveraged to improve water quality and corrosion management programs. Results from QuantArray®-BGC, QuantArray®-Petro, and Microbial Source Tracking can inform water reuse practices in constructed wetlands and municipal and industrial wastewater treatment. Likewise, QuantArray®-MIC and next generation sequencing can provide important information for microbiologically influenced corrosion assessment.
This brochure explores how microbial testing can aid landfill management. Several microbial processes, including hydrolysis, fermentation, acetogenesis, and methanogenesis, are important to landfill functioning and can be monitored using QuantArray®-BGC and next generation sequencing.