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Oil Reservoir Souring and Microorganisms White Paper

This white paper explains how sulfate reducing microorganisms contribute to oil reservoir souring and offers strategies for mitigating reservoir souring. Molecular biological tools like qPCR, next generation sequencing, and whole genome sequencing can be used to monitor microorganisms that are important for oil reservoir souring and souring mitigation.

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Wastewater Treatment and Molecular Biological Tools White Paper

Molecular biological tools offer innovative solutions for monitoring microbial communities that play essential roles in wastewater treatment. This white paper explores how qPCR, next generation sequencing, and whole genome sequencing can be used to monitor microorganisms that are important for anaerobic digester management, nitrogen cycling, enhanced biological phosphorus removal, bulking and foaming, and microbial source…

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1,4-Dioxane Solutions

This handout describes how microbial monitoring at sites impacted with 1,4-dioxane can help identify biodegradation potential, support remediation design, and improve regulatory compliance and risk assessment. Microbial analyses that support 1,4-dioxane bioremediation projects include CENSUS® qPCR, stable isotope probing, and whole genome sequencing.

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Microbial Scale Formation and Dissolution White Paper

This paper provides an overview of microorganisms involved in bioscale production and dissolution, which can cause issues in a variety of industries, including oil and gas, mining, and industrial heating systems. It also highlights how molecular biological tools such as qPCR (CENSUS®), next generation sequencing and whole genome sequencing enable effective monitoring and mitigation of…

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CENSUS® qPCR: Evaluating Biostimulation to Overcome DCE Stall

A site impacted by PCE and TCE was experiencing an accumulation of cis-DCE (DCE stall). CENSUS qPCR quantification demonstrated that Dehalococcoides concentrations were too low for effective rates of complete reductive dechlorination. Site managers asked: “Is bioaugmentation required or will biostimulation alone be sufficient to result in complete reductive dechlorination?” Monitoring with Bio-Traps® and CENSUS® analysis showed that stimulation…

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CENSUS® qPCR: Actionable Data for Evaluating MNA

A petroleum pipeline release in a remote area had gone undiscovered for a number of years and groundwater was impacted with BTEX. Groundwater monitoring results suggested that BTEX concentrations were decreasing although results were variable. The site managers asked for an additional line of evidence of BTEX biodegradation to support acceptance of an MNA management…

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ISMs: Cost Effective Remedy Selection at a TCE Impacted Site

At a site impacted by trichloroethene (TCE), biostimulation and bioaugmentation were considered as remediation strategies. The site managers asked: “Is bioaugmentation required at this site for meaningful rates of reductive dechlorination?” The answer was no.  An In Situ Microcosm (ISM) study showed conclusively that Dehalococcoides (DHC) was present at the site at low levels but could be significantly stimulated by…

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NGS Characterization of Microbial Communities at a Pipeline Release

At a petroleum pipeline release in a remote area Bio-Traps® and CENSUS® qPCR for BTEX catabolic functional genes proved stimulation of BTEX biodegradation in impacted groundwater and provided an additional line of evidence to support acceptance of an MNA management strategy at the site. Site managers asked: “Can molecular biological tools provide even more evidence…