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CENSUS® qPCR: Evaluating Biostimulation to Overcome DCE Stall

A site impacted by PCE and TCE was experiencing an accumulation of cis-DCE (DCE stall). CENSUS qPCR quantification demonstrated that Dehalococcoides concentrations were too low for effective rates of complete reductive dechlorination. Site managers asked: “Is bioaugmentation required or will biostimulation alone be sufficient to result in complete reductive dechlorination?” Monitoring with Bio-Traps® and CENSUS® analysis showed that stimulation…

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CENSUS® qPCR: Actionable Data for Evaluating MNA

A petroleum pipeline release in a remote area had gone undiscovered for a number of years and groundwater was impacted with BTEX. Groundwater monitoring results suggested that BTEX concentrations were decreasing although results were variable. The site managers asked for an additional line of evidence of BTEX biodegradation to support acceptance of an MNA management…

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ISMs: Cost Effective Remedy Selection at a TCE Impacted Site

At a site impacted by trichloroethene (TCE), biostimulation and bioaugmentation were considered as remediation strategies. The site managers asked: “Is bioaugmentation required at this site for meaningful rates of reductive dechlorination?” The answer was no.  An In Situ Microcosm (ISM) study showed conclusively that Dehalococcoides (DHC) was present at the site at low levels but could be significantly stimulated by…

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Combining In Situ Microcosms and Stable Isotope Probing

A site impacted by chlorobenzene was managed by MNA. Site managers asked: “Can we confirm that observed decreases in chlorobenzene concentrations are resulting from biodegradation as opposed to dilution?” and “Would injection of an oxygen release compound result in enhanced rates of aerobic biodegradation of chlorobenzene under site conditions?” The answers to both questions were yes. …

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SIP: Evaluating MNA at a Manufactured Gas Plant

Site stakeholders needed conclusive evidence that biodegradation of benzene and naphthalene was occurring under existing site conditions to accept MNA instead of a more aggressive strategy. Stable isotope probing (SIP) studies using Bio-Traps® amended with 13C benzene or 13C naphthalene conclusively demonstrated in situ biodegradation occurred under existing site conditions. MNA was accepted as the remediation strategy.

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In Situ Microcosms MLOE

This brochure provides an overview of how In Situ Microcosm studies are designed to cost-effectively collect multiple lines of evidence to evaluate remedial amendments under actual aquifer conditions. Comparisons between In Situ Microcosms, bench scale studies, and pilot studies are also included.

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In Situ Microcosm Brochure

This product brochure provides an overview of how Bio-Trap® In Situ Microcosms (ISMs) provide microbial, chemical, and geochemical evidence for simultaneous, cost-effective evaluation of multiple remediation alternatives (e.g., MNA, Biostimulation, Bioaugmentation).

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Top Tips – Evaluating Remediation Options – Petroleum

This Top Tips bulletin explains how molecular biological tools can be used during site assessment to evaluate monitored natural attenuation (MNA), enhanced bioremediation, and in situ chemical oxidation (ISCO) at petroleum‑impacted sites. It highlights how in situ microcosms, QuantArray®‑Petro, CENSUS® qPCR, and stable isotope probing provide actionable, field‑based evidence to compare remedies and select the…

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Top Tips to Save Money with MNA

This Top Tips handout explains how monitored natural attenuation (MNA) can be a cost‑effective site management strategy at petroleum‑impacted sites when supported by multiple lines of evidence. It highlights how molecular biological tools, stable isotope probing, and in situ microcosms provide defensible proof of biodegradation and help compare MNA with enhanced remediation options to reduce…