Understanding and Addressing Reservoir Souring: Costs, Challenges, and Solutions Reservoir souring is a common and costly challenge in the oil and gas industry, especially during secondary recovery processes. This phenomenon is primarily caused by the activity of sulfate-reducing bacteria (SRB) and sulfate-reducing archaea (SRA), which metabolize sulfate ions in water to produce hydrogen sulfide (H₂S).…
Unveiling Microbial Insights: The Power of Next Generation Sequencing (NGS) Today, we’re diving into the realm of Next Generation Sequencing (NGS), a cutting-edge technique that has revolutionized our understanding of microbial communities. NGS is transforming the field of microbiology and shedding light on intricate processes like Environmental Remediation (ER) and Microbiologically Influenced Corrosion (MIC). NGS…
– This case study shows how NGS revealed diverse microbial communities and the presence of microbial groups commonly implicated in MIC, including SRB, methanogens and acetogens.
• A pilot study was conducted at a site where electron donor was injected into groundwater impacted by a mixture of chlorinated solvents.
• QuantArray®-Chlor and NGS provided complimentary evidence of both biotic and abiotic reductive dechlorination in response to the injection of the electron donor giving the client confidence in this remediation strategy expanding electron donor injection to the entire site.
• At a petroleum pipeline release in a remote area Bio-Traps® and CENSUS® qPCR for BTEX catabolic functional genes proved stimulation of BTEX biodegradation in impacted groundwater and provided an additional line of evidence to support acceptance of an MNA management strategy at the site.
• Contaminant trends, geochemistry, CENSUS® qPCR, and NGS provided multiple lines of evidence supporting an MNA management strategy and avoiding the greater costs of enhanced remediation.