Next generation sequencing (NGS) provides comprehensive identification of microorganisms present in a sample down to the genus and even species level.

Next Generation Sequencing (NGS): Characterization of Microbial Communities at a Pipeline Release

• At a petroleum pipeline release in a remote area Bio-Traps® and CENSUS® qPCR for BTEX catabolic functional genes proved stimulation of BTEX biodegradation in impacted groundwater and provided an additional line of evidence to support acceptance of an MNA management strategy at the site.

• Contaminant trends, geochemistry, CENSUS® qPCR, and NGS provided multiple lines of evidence supporting an MNA management strategy and avoiding the greater costs of enhanced remediation.

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QuantArray®-Petro: Effectiveness of Oxygen Addition at a Former Gas Station

• Groundwater at a former gasoline service station was impacted by leaking underground storage tanks and associated piping. Although contaminant trends and geochemistry suggested that MNA could be an appropriate site management strategy, the need for more rapid site closure led to a decision to enhance biodegradation of BTEX and MTBE aerobically with injection of an oxygen releasing compound.

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CENSUS® qPCR: Actionable Data for Evaluating MNA

• A petroleum pipeline release in a remote area had gone undiscovered for a number of years and groundwater was impacted with BTEX. Groundwater monitoring results suggested that BTEX concentrations were decreasing although results were variable.

• Trends in decreasing BTEX concentrations were validated as biological in origin supporting an MNA management strategy and avoiding the greater costs of enhanced remediation.