At a petroleum pipeline release in a remote area Bio-Traps® and CENSUS® qPCR for BTEX catabolic functional genes proved stimulation of BTEX biodegradation in impacted groundwater and provided an additional line of evidence to support acceptance of an MNA management strategy at the site. Site managers asked: “Can molecular biological tools provide even more evidence…
A pilot study was conducted at a site where electron donor was injected into groundwater impacted by a mixture of chlorinated solvents. Site managers asked two questions: “Did the electron donor injection stimulate reductive dechlorination?” and “Did injection of the electron donor also increase the potential for abiotic dechlorination through the generation of biogenic FeS?”…
Next generation sequencing (NGS) was performed on liquid samples from key locations with different operating conditions. NGS revealed diverse microbial communities and the presence of microbial groups commonly implicated in MIC including sulfate reducing bacteria (SRB), methanogens, and acetogens. In conjunction with a review of operating conditions, corrosion monitoring, and inspections, the NGS results added a…
This brochure provides an overview of the molecular microbiological methods (MMMs) relevant to addressing microbiologically influenced corrosion (MIC) in the context of midstream oil and gas operations. Relevant applications for CENSUS® qPCR, QuantArray®-MIC and next generation sequencing are discussed.
This article offers an overview of how to leverage molecular biological tools to evaluate and enhance petroleum hydrocarbon remediation. It provides practical guidance and field case studies for a variety of microbial analyses, including quantitative polymerase chain reaction (qPCR), qPCR arrays, RT-qPCR, stable isotope probing (SIP), targeted taxonomic sequencing, and metagenome sequencing.
This open access book synthesizes important advances in the assessment and management of soil and groundwater systems contaminated with petroleum hydrocarbons. Chapter 10: Molecular Biological Tools Used in Assessment and Remediation of Petroleum Hydrocarbons in Soil and Groundwater is co-authored by Dora M. Taggart and Trent A. Key.
This white paper provides an overview of important types of biofuels, their production methods, and associated environmental concerns. CENSUS® qPCR and next generation sequencing can be used to monitor biofuel systems and assess the biodegradation of biofuels.
This white paper provides an overview of important interactions that can occur between thermophilic microorganisms and industrial materials, including biofouling, bioscaling, and microbiologically influenced corrosion. Next generation sequencing can be used to monitor thermophilic microorganisms.
This brochure examines how MBTs can be leveraged to improve water quality and corrosion management programs. Results from QuantArray®-BGC, QuantArray®-Petro, and Microbial Source Tracking can inform water reuse practices in constructed wetlands and municipal and industrial wastewater treatment. Likewise, QuantArray®-MIC and next generation sequencing can provide important information for microbiologically influenced corrosion assessment.
This brochure explores how data from molecular biological tools can enhance the efficiency, sustainability, and environmental responsibility of mining operations. CENSUS® qPCR, next generation sequencing, and assay development can be applied to three key areas: acid rock drainage, bioleaching and rare earth element (REE) recovery, and bioprospecting.