At a site impacted by trichloroethene (TCE), biostimulation and bioaugmentation were considered as remediation strategies. The site managers asked: “Is bioaugmentation required at this site for meaningful rates of reductive dechlorination?” The answer was no. An In Situ Microcosm (ISM) study showed conclusively that Dehalococcoides (DHC) was present at the site at low levels but could be significantly stimulated by…
A site impacted by chlorobenzene was managed by MNA. Site managers asked: “Can we confirm that observed decreases in chlorobenzene concentrations are resulting from biodegradation as opposed to dilution?” and “Would injection of an oxygen release compound result in enhanced rates of aerobic biodegradation of chlorobenzene under site conditions?” The answers to both questions were yes. …
An In Situ Microcosm study was conducted to evaluate MNA vs. enhanced anaerobic bioremediation via sulfate reduction at a petroleum impacted site. Site managers asked: “Will the injection of sulfate stimulate anaerobic BTEX biodegradation at this site?” The answer was yes. The ISM study showed conclusively that injection of sulfate at the site would increase the growth of…
An In Situ Microcosm study was conducted to evaluate MNA vs. enhanced aerobic bioremediation at a petroleum impacted site. Site managers asked: “Will the injection of an oxygen releasing compound stimulate BTEX biodegradation at this site?” The answer was yes. The ISM study showed conclusively that injection of the an oxygen releasing compound at the site will increase…
This brochure provides guidance on selecting appropriate molecular biological tools, including QuantArray®, CENSUS® qPCR, SIP, the abiotic panel, and in situ microcosms, based on the chlorinated compounds present and the chosen remedial approach.
This brochure provides guidance on selecting appropriate molecular biological tools, including QuantArray®, CENSUS® qPCR, SIP, and in situ microcosms, based on the petroleum hydrocarbon contaminants present and the chosen remedial approach.
This brochure provides an overview of how In Situ Microcosm studies are designed to cost-effectively collect multiple lines of evidence to evaluate remedial amendments under actual aquifer conditions. Comparisons between In Situ Microcosms, bench scale studies, and pilot studies are also included.
This product brochure provides an overview of how Bio-Trap® In Situ Microcosms (ISMs) provide microbial, chemical, and geochemical evidence for simultaneous, cost-effective evaluation of multiple remediation alternatives (e.g., MNA, Biostimulation, Bioaugmentation).
This Top Tips bulletin explains how molecular biological tools can be used during site assessment to evaluate monitored natural attenuation (MNA), enhanced bioremediation, and in situ chemical oxidation (ISCO) at petroleum‑impacted sites. It highlights how in situ microcosms, QuantArray®‑Petro, CENSUS® qPCR, and stable isotope probing provide actionable, field‑based evidence to compare remedies and select the…
This Top Tips handout explains how monitored natural attenuation (MNA) can be a cost‑effective site management strategy at petroleum‑impacted sites when supported by multiple lines of evidence. It highlights how molecular biological tools, stable isotope probing, and in situ microcosms provide defensible proof of biodegradation and help compare MNA with enhanced remediation options to reduce…