Industrial & Municipal Wastewater Testing & Analysis

Microbial Insights has been a leader in the industry, offering cutting edge technologies for the testing and analysis of industrial & municipal wastewater. Our molecular biological tools (MBTs) provide more accurate quantification of key microorganisms like nitrifiers and comprehensive characterization of microbial communities to identify problematic microorganisms, giving you the crucial information needed to make informed operating decisions.

ISSUE: Industrial and municipal wastewater treatment depends upon having the “right” microorganisms present at sufficient concentrations for optimal operation. Conversely, problematic organisms can lead to foaming, bulking, poor settling and other issues.

IMPACT: Influent characteristics, surges, shocks, and operating conditions will impact the microbial community, concentrations of desired and problematic microorganisms, and ultimately plant operation and performance.

THREATS: Exceeding discharge limits + Plant upsets.

PATH TO RESOLUTION: Microbial Insights’ DNA based methods can cost-effectively answer microbiological questions which cannot be answered with microscopy or other traditional methods.

Industrial & municipal wastewater

THE MI ADVANTAGE

Explore Our Molecular Biological Tools (MBTs) and Site Logic Services

Nitrogen: Quantify microorganisms or functional genes responsible for processes in the nitrogen cycle including nitrification, denitrification, and anaerobic ammonia oxidation (Anammox), including Ammonia Oxidizing Bacteria (AMO), Nitrite Oxidizing Bacteria (NOB, NOR), Ammonia Oxidizing Archaea (AOA), Denitrifying Bacteria (DNF), Archaeal Nitrate Reducers (ADNF), and Anaerobic Ammonia Oxidizing Bacteria (AMX).

Closing the Nitrogen Cycle with MBTs Resource Sheet

CENSUS® (qPCR) ER

Phosphorus: Determine if potential polyphosphate-accumulating organisms (PAOs) and competing glycogen-accumulating organisms (GAOs) are present. NGS results provide relative abundances of detected genera as % of total reads.

Next Generation Sequencing (NGS) ER

Filamentous Organisms & Sludge Bulking: Determine if common sludge bulking bacteria such as Microthrix, Sphaerotilus and Thiothrix are present.

Next Generation Sequencing (NGS) ER

Anaerobic Digestion: Quantify concentrations of methanogens (MGN) responsible for methane (biogas) generation and the last step in anaerobic digestion. Or use Next Generation Sequencing (NGS) to examine the overall bacterial and archaeal communities present in an anaerobic digester.

CENSUS® (qPCR) ER

RESOURCES

Wastewater and Feedstock Analysis Handout

Closing the Nitrogen Cycle with MBTs Handout

AVAILABLE CENSUS® qPCR ASSAYS RELATED TO THE NITROGEN CYCLE

TARGET	MI CODE	RELEVANCE / DATA INTERPRETATION
Ammonia Oxidizing Bacteria	AMO	The AMO assay targets the ammonia monooxygenase gene (amoA) that encodes the enzyme responsible for the initial oxidation of ammonia in the nitrification process.
Ammonia Oxidizing Archaea	AOA	Taxonomic gene for archaea that can oxidize ammonia to nitrite.
Nitrite Oxidizing Bacteria	NOB	Responsible for converting nitrite to nitrate.
Nitrite Oxidizing Bacteria	NOR/NXR	Targets the gene encoding the enzyme responsible for the last step in nitrification.
Denitrifying Bacteria	DNF	The assays target the genes encoding two types of nitrite reductase enzymes (nirS and nirK) for quantification of denitrifying bacteria.
Archaeal Nitrate Reducers	ADNFNIRK	Functional gene responsible for converting nitrite to nitric oxide in archaeal organisms.
Archaeal Nitrate Reducers	ADNFNIRS	Functional gene responsible for converting nitrite to nitric oxide in archaeal organisms.
Anaerobic Ammonia Oxidizing Bacteria	AMXNIRS	This assay targets a nitrite reductase gene involved in converting nitrite to nitric oxide.
Anaerobic Ammonia Oxidizing Bacteria	AMXNIRK	This assay targets a nitrite reductase gene involved in converting nitrite to nitric oxide.
Nitrogen Fixing Bacteria	NIF	Targets the gene nifD related to the conversion of nitrogen to ammonium which can be assimilated by organisms.

EASY STEPS TO ADDRESS YOUR INDUSTRIAL & MUNICIPAL WASTEWATER NEEDS

1) Call us at 865.573.8188 or email at [email protected]
2) A friendly member of our team will advise you on the right tools for the job
3) Order and receive your supplies
4) Perform sampling according to our clear and easy protocols
5) Fill out the chain of custody form
6) Send to the nearest Microbial Insights location
7) Receive your unbiased report with actionable data and insight

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