Microbial Insights has extensive expertise in environmental microbiology and providing remediation strategies for the agriculture industry. We offer highly accurate, cost-effective, and customizable molecular biological tools (MBTs) to provide the comprehensive data needed to help agri-businesses, consultants, and government agencies make informed and effective agricultural management, environmental assessment, and remediation decisions.

Identifying Microbial Threats to Agriculture

Agriculture relies on a complex relationship between microbial, fungal, plant and animal communities in addition to the specifics of a given piece of land and the climate of the region it inhabits.  The interplay of these relationships creates significant room for both threats and opportunities to emerge.  As one of the communities that is impacted by changes the fastest, microbial communities have the unique ability to provide early warning signs about the health of the overall system. Threats can come from many areas, including: Accidental Spills, Bacterial or Fungal Infections, waste and nutrient contamination and changes in seasonal patterns of precipitation and heat.

Impacts & Threats

The impacts of the above threats can be felt in reduced productive power of both the land and the animals on it in addition to loss of healthy crops and livestock.  The microbial community can act as advanced warning, cause or solution of a wide array of potential issues.  Poor soil health or soil ecology with a poor mismatch with desired crops can significantly diminish the returns on and sustainability of farmland.

Our Molecular Testing Methods

Our superior molecular biological tools and data interpretation provide you with the detailed insight you need to make informed decisions that impact agricultural management and environmental remediation. Genomics and other DNA based methods can cost-effectively answer microbiological questions that are simply not possible with other methods. Our analysis is 100% unbiased. Microbial Insights does not sell biologicals or remediation products.  We provide the MBTs and reporting that help you make the most informed decisions, based on the best possible data and analysis.  

Research is showing that taking into account the entire ecology of a location is one of the best ways to ensure durable production that can weather changes in environment and disease while maximizing overall productivity.  Understanding microbial communities can be a vital piece of that puzzle.  In addition, microbes can either be destructive pests or beneficial helpers, our tools can help you make sure they are productive partners and not sources of frustration.

Agriculture INDUSTRY

THE MI ADVANTAGE

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Explore Our Molecular Biological Tools (MBTs) and Site Logic Services

Nutrients and Plant Growth Promoting Rhizobacteria (PGPR) For absolute quantification of microorganisms or functional genes responsible for processes in the nitrogen cycle including nitrogen fixation, nitrification, and denitrification, including Nitrogen Fixing Bacteria (NIF), Ammonia Oxidizing Bacteria (AMO), and Denitrifying Bacteria (DNF).

Nutrients and Plant Growth Promoting Rhizobacteria (PGPR) Generate an overall profile of the rhizosphere microbial community and determine if potential nitrogen fixing, phosphate solubilizing, or siderophore producing bacteria are present.  NGS can also reveal the presence of nitrifying and denitrifying bacteria.  NGS results provide relative abundances of detected genera as % of total reads.

Pesticides Examine the microbial community for bacteria associated with degradation of common pesticides.

Fungal:Bacterial (F:B) Biomass Ratios. This ratio has been linked to overall soil health, carbon cycling, and nutrient usage. In general, higher soil fertility with fewer disturbances results in a higher percentage of fungal biomass. Phospholipid Fatty Acid (PLFA) analysis provides a reliable F:B biomass ratio for soil samples.

RELATED TOPICS

Environmental Remediation Remediation needs related to Agriculture can spring from any number of areas: Contaminated well water, a third party accidental spill on your land, human and animal contaminants from upstream, etc.  Identifying and resolving these will help ensure the long term health and productivity of your farmland.

Microbial Source Tracking Solving a problem first requires understanding both its nature and its source.  Microbial Source Tracking tools allow you to differentiate between hosts to better identify the source of an issue, letting you take more appropriate remedial or preventative measures.  As not all hosts are of equal concern, this can allow you to better match your strategy to the risk being posed.

Biofuels From fermentation in current biofuel production to advanced biofuels research to ensuring the quality of output, Biofuels are heavily dependent on the presence and activity desired of target microorganisms.  MI’s MBTs provide greater clarity and insight so that you can achieve consistent quality and safely maintain your product during storage.

RESOURCES

AVAILABLE CENSUS® qPCR ASSAYS RELATED TO THE NITROGEN CYCLE

 

TARGETMI CODERELEVANCE / DATA INTERPRETATION
Ammonia Oxidizing BacteriaAMOThe AMO assay targets the ammonia monooxygenase gene (amoA) that encodes the enzyme responsible for the initial oxidation of ammonia in the nitrification process.
Ammonia Oxidizing ArchaeaAOATaxonomic gene for archaea that can oxidize ammonia to nitrite.
Nitrite Oxidizing BacteriaNOBResponsible for converting nitrite to nitrate.
Nitrite Oxidizing BacteriaNOR/NXRTargets the gene encoding the enzyme responsible for the last step in nitrification.
Denitrifying BacteriaDNFThe assays target the genes encoding two types of nitrite reductase enzymes (nirS and nirK) for quantification of denitrifying bacteria.
Archaeal Nitrate ReducersADNFNIRKFunctional gene responsible for converting nitrite to nitric oxide in archaeal organisms.
Archaeal Nitrate ReducersADNFNIRSFunctional gene responsible for converting nitrite to nitric oxide in archaeal organisms.
Anaerobic Ammonia Oxidizing BacteriaAMXNIRSThis assay targets a nitrite reductase gene involved in converting nitrite to nitric oxide.
Anaerobic Ammonia Oxidizing BacteriaAMXNIRKThis assay targets a nitrite reductase gene involved in converting nitrite to nitric oxide.
Nitrogen Fixing BacteriaNIFTargets the gene nifD related to the conversion of nitrogen to ammonium which can be assimilated by organisms.

EASY STEPS TO ADDRESS YOUR AGRICULTURAL NEEDS

1) Call us at 865.573.8188 or email at [email protected]
2) A friendly member of our team will advise you on the right tools for the job
3) Order and receive your supplies
4) Perform sampling according to our clear and easy protocols
5) Fill out the chain of custody form
6) Send to the nearest Microbial Insights location
7) Receive your unbiased report with actionable data and insight

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