In-situ enrichment and analysis of atrazine-degrading microbial communities using atrazine-containing porous beads
Dhritiman Ghosh1, Krishnakali Roy1, Velusamy Srinivasan1, Tom Mueller2, Olli H. Tuovinen3, Kerry Sublette4, Aaron Peacock1, and Mark Radosevich1
Abstract
We examined the community composition of microbes that colonized atrazine-containing beads buried in agricultural soils that differed in atrazine treatment history. Bacterial abundance was 5–40-fold greater in atrazine-fortified beads. In beads containing 20 mg atrazine kg−1 buried in soil with a history of atrazine application (conditioned soil), the abundance of Actinobacteria increased approximately 80-fold whereas in control soil, Actinobacteria were enriched only 10-fold and the gamma-Proteobacteria and Planctomycetes increased by 60- and 25-fold, respectively. The gamma-Proteobacteria were enriched by 120- and 230-fold in beads containing 200 mg atrazine kg−1 in conditioned and control soil, respectively. The results demonstrate that BioSep® beads are a suitable matrix for recruiting a diverse subset of the bacterial community involved in atrazine degradation.
1 Biosystems Engineering and Soil Science, University of Tennessee, 2 Plant Sciences Department, University of Tennessee, 3 Department of Microbiology, The Ohio State University, 4 Chemical Engineering Department, University of Tulsa
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